The Distribution of Lipoprotein Lipase in Rat Adipose Tissue

Purification and characterization of rat adipose tissue lipoprotein lipase.

Lipoprotein lipase (EC 3.1.1.34) extracted from adipose tissue of glucose-fed rats with 5 mM-sodium barbital, pH 7.5, containing 20% (v/v) glycerol and 0.1% (v/v) Triton X-100, was partially purified by affinity chromatography on heparin linked to Sepharose 4B. Sodium dodecyl sulphate/polyacrylamide-gel electrophoresis of the partially purified enzyme preparation revealed the presence of two ma...

Lipoprotein lipase in human adipose tissue.

Determinants of Human Adipose Tissue Lipoprotein Lipase

to percent of ideal body weight (r = 0.76, P < 0.001 for controls; r = 0.67, P <0.05 for diabetics) and to fat cell size (r = 0.71, P <0.01 for controls; r = 0.85, P <0.01 for diabetics. Postprandial-stimulated insulin secretion was related to diet-induced changes in lipoprotein lipase in control subjects; both were dependent upon the amount of dietary carbohydrate. In contrast, the diabetic pa...

Lipoprotein lipase of chicken adipose tissue.

In recent publications (l-3), the clearing factor of postheparin plasma has been identified with a lipoprotein lipase obtained from rat, rabbit, and calf heart and rat and rabbit adipose tissue. This enzyme catalyzes the hydrolysis of the triglyceride moiety of chylomicrons and low density @-lipoproteins. The hydrolysis of simple triglyceride emulsions is also catalyzed by lipoprotein lipase bu...

Increase of Adipose Tissue Lipoprotein Lipase

A B S T R A C T Obese subjects have elevated adipose tissue lipoprotein lipase activity per fat cell when compared with lean control subjects. This enzyme, which is rate limiting for the uptake and storage of lipoprotein triglyceride in adipose tissue, has been shown to be further elevated in a group ofpreviously obese subjects who had been weight stable at a reduced weight for 428 mo. In the p...